HIGH FREQUENCY TRANSDUCTION OF GALACTOSE MARKERS BY PHAGE φ 170

High frequency generalized transduction by miniMu plasmid phage.

Deletion derivatives of phage Mu which replicate as multicopy plasmids, and also transpose and package like Mu, have been developed for the in vivo cloning of bacterial genes. We show here that these miniMu plasmid phage are also efficient at generalized transduction and that both in vivo cloning and generalized transduction of a given gene can be accomplished in a single experiment.

φ Bacteriophage Lactobacillus gasseri High - Frequency Plasmid Transduction

High frequency of a novel filamentous phage, VCY φ, within an environmental Vibrio cholerae population.

Environmental Vibrio cholerae strains isolated from a coastal brackish pond (Oyster Pond, Woods Hole, MA) carried a novel filamentous phage, VCY, which can exist as a host genome integrative form (IF) and a plasmid-like replicative form (RF). Outside the cell, the phage displays a morphology typical of Inovirus, with filamentous particles ∼1.8 μm in length and 7 nm in width. Four independent RF...

Specialized transduction of tetracycline resistance by phage P22 in Salmonella typhimurium. II. Properties of a high-frequency-transducing lysate.

A high-frequency-transducing (HFT) lysate for tetracycline resistance (Lets) was obtained by inducing an unusual tets P22 lysogen which had been made by transducing Salmonella typhimurium LT.2 to letR with P22 phage grown on an LT-2 strain carrying the R factor 222. The HFT lysate contains defective P22 particles, called P22 Tc-10, which cannot grow or lysogenize (i.e., transduce tetn) upon sin...

Specialized transduction of tetracycline resistance by phage P22 in Salmonella typhimurium. I. Transduction of R factor 222 by phage P22.

Transduction of R factor 222, which carries four drug resistance markers (~0, strR, camR, totR), by phage P22 in Salmonella typhimurium results in the segregation of the drug resistance markers into tetR and the other three. The tetR marker was found to be integrated at proA near the P22 prophage attachment site of the recipient chromosome, confirming the previous result of Dubnau and Stocker (...